Category Archives: Publications

An analysis of the resolution limitations of peptide identification algorithms

Proteome identification using peptide-centric proteomics techniques is a routinely used analysis technique. One of the most powerful and popular methods for the identification of peptides from MS/MS spectra is protein database matching using search engines. Significance thresholding through false discovery … Continue reading

Posted in 2011, Publications

jTraML: An Open Source Java API for TraML, the PSI Standard for Sharing SRM Transitions

We here present jTraML, a Java API for the Proteomics Standards Initiative TraML data standard. The library provides fully functional classes for all elements specified in the TraML XSD document, as well as convenient methods to construct controlled vocabulary-based instances … Continue reading

Posted in 2011, Publications

Bioinformatics challenges in the proteomic analysis of human plasma

Mass spectrometry has become the method of choice for studying proteins in complex mixtures in a qualitative and quantitative fashion. The application of mass spectrometry-based proteomics analyses on plasma has correspondingly been established as an important method for disease-associated biomarker … Continue reading

Posted in 2011, Publications

Bioinformatics challenges in mass spectrometry-driven proteomics

Mass spectrometry-based proteomics has become an essential part of the analytical toolbox of the life sciences. With the ability to identify and quantify hundreds to thousands of proteins in high throughput, the field has contributed its fair share to the … Continue reading

Posted in 2011, Publications

thermo-msf-parser: an open source Java library to parse and visualize Thermo Proteome Discoverer msf files

The Thermo Proteome Discoverer program integrates both peptide identification and quantification into a single workflow for peptide-centric proteomics. Furthermore, its close integration with Thermo mass spectrometers has made it increasingly popular in the field. Here, we present a Java library … Continue reading

Posted in 2011, Publications

Bioinformatics analysis of a Saccharomyces cerevisiae N-terminal proteome provides evidence of alternative translation initiation and post-translational N-terminal acetylation.

Initiation of protein translation is a well studied fundamental process, albeit high-throughput and more comprehensive determination of the exact translation initiation sites (TIS) was only recently made possible following the introduction of positional proteomics techniques that target protein N-termini. Precise … Continue reading

Posted in 2011, Publications

RIBAR and xRIBAR: Methods for Reproducible Relative MS/MS-based Label-Free Protein Quantification.

Mass spectrometry-driven proteomics is increasingly relying on quantitative analyses for biological discoveries. As a result, different methods and algorithms have been developed to perform relative or absolute quantification based on mass spectrometry data. One of the most popular quantification methods … Continue reading

Posted in 2011, Publications